Impact of Pure Culture Storage Techniques on the Endoglucanase Activity in Macrofungi

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The paper focuses on analyzing how different storage methods affect the endoglucanase activity of pure macrofungal cultures. A total of 20 macrofungal species from diverse taxonomic and eco-trophic groups were examined. Five storage techniques were employed: serial transfers, storage beneath a layer of distilled water, and three cryopreservation protocols – one involving agar medium blocks, another using perlite, and the third employing grain-based methodology. For cryopreservation, various cryoprotective agents such as glycerol and trehalose were utilized. Upon retrieval of the cultures from storage, their endoglucanase activity was assessed. Results revealed that the highest levels of endoglucanase activity were observed in cultures stored under distilled water and following the “grain protocol”.

作者简介

N. Komissarov

Lomonosov Moscow State University

Email: macoloams@gmail.com
119234 Москва, Россия

M. Dyakov

Lomonosov Moscow State University

Email: max_fungi@mail.ru
119234 Москва, Россия

I. Sidorova

Lomonosov Moscow State University

Email: irsidor2008@yandex.ru
119234 Москва, Россия

参考

  1. Ahlawat O. P., Manikandan K., Singh M. Proximate composition of different mushroom varieties and effect of UV light exposure on vitamin D content in Agaricus bisporus and Volvariella volvacea. Mushroom Res. 2016. V. 25 (1). P. 1–8.
  2. Antropova A. B., Belozerskaya T. A., Belozersky M. A. et al. Practical training in the physiology and biochemistry of fungi. A tutorial. MSU, Moscow. (In Russ.).
  3. Balaeş T., Tănase C., Butnariu C. The use of heavy metals in mycoremediation of synthetic dyes. Open Life Sci. 2014. V. 9 (7). P. 659–667. https://doi.org/10.2478/s11535-014-0302-5
  4. Baldrian P. Wood-inhabiting ligninolytic basidiomycetes in soils: ecology and constraints for applicability in bioremediation. Fungal Ecol. 2008. V. 1 (1). P. 4–12. https://doi.org/10.1016/j.funeco.2008.02.001
  5. Baldrian P., Valášková V. Degradation of cellulose by basidiomycetous fungi. FEMS Microbiol. Rev. 2008. V. 32 (3). P. 501–521.
  6. Bertéli M. B.D., Pinheiro C. R., Philadelpho B. O. et al. Long-term cryopreservation of Lentinus crinitus strains by wheat grain technique. J. Microbiological Methods. 2022. V. 198. https://doi.org/10.1016/j.mimet.2022.106491
  7. Bukhalo A. S. Higher edible basidiomycetes in pure culture. Naukova Dumka, Kiev, 1988. (In Russ.).
  8. Burdsall H. H. Jr., Dorworth E. B. Preserving cultures of wood-decaying Basidiomycotina using sterile distilled water in cryovials. Mycologia. 1994. V. 86 (2). P. 275–280. https://doi.org/10.1080/00275514.1994.12026408
  9. Colauto N. B., da Eira A. F., Linde G. A. Cryopreservation at –80 °C of Agaricus blazei on rice grains. World J. Microbiol. Biotechnol. 2011. V. 27 (12). P. 3015–3018. https://doi.org/10.1007/s11274-011-0772-9
  10. Deshmukh R., Khardenavis A. A., Purohit H. J. Diverse metabolic capacities of fungi for bioremediation. Indian J. Microbiol. 2016. V. 56 (3). P. 247–264. https://doi.org/10.1007/s12088-016-0584-6
  11. Furlani R. P.Z., Godoy H. T. Vitamins B1 and B2 contents in cultivated mushrooms. Food Chemistry. 2008. V. 106 (2). P. 816–819. https://doi.org/10.1016/j.foodchem.2007.06.007
  12. Homolka L., Lisá L., Eichlerová I. et al. Cryopreservation of basidiomycete strains using perlite. J. Microbiol. Methods. 2001. V. 47 (3). P. 307–313. https://doi.org/10.1016/S0167-7012(01)00338-4
  13. Homolka L., Lisa L., Kubátová A. et al. Cryopreservation of filamentous micromycetes and yeasts using perlite. Folia Microbiologica. 2007. V. 52 (2). P. 153–157. https://doi.org/10.1007/BF02932154
  14. Hutchinson H. B., Clayton J. On the decomposition of cellulose by an aerobic organism (Spirochaeta cytophaga, n. sp.). J. Agric. Sci. 1919. V. 9 (2). P. 143–172. https://doi.org/10.1017/S0021859600004755
  15. Hwang S. W. Effects of ultra-low temperatures on the viability of selected fungus strains. Mycologia. 1960. V. 52 (3). P. 527–529. https://doi.org/10.2307/3755974
  16. Keuls M. The use of the “studentized range” in connection with an analysis of variance. Euphytica. 1952. V. 1. P. 112–122. https://doi.org/10.1007/BF01908269
  17. Komissarov N. S., Dyakov M. Yu., Garibova L. V. Methods of long-term storage of pure cultures of macromycetes. Mikologiya i fitopatologiya. 2023. V. 57 (3). P. 155–171. (In Russ.). https://doi.org/10.31857/S0026364823030054
  18. Kovalenko A. E. Ecological review of fungi from the orders Polyporales s.str., Boletales, Agaricales s.str., Russulales in the mountain forests of the central part of the North-West Caucasus. Mikologiya i fitopatologiya. 1980. V. 14 (4). P. 300–314. (In Russ.).
  19. Kovalenko S. A. Collection fund of basidiomycete strains of the Institute of Forest of the National Academy of Sciences of Belarus. Problems of forest phytopathology and mycology: Proceedings of the XI international conference, Petrozavodsk, October 10–14, 2022. Karelian Research Center of the Russian Academy of Sciences, Petrozavodsk, 2022, pp. 26–28. (In Russ.).
  20. Kulikova N. A., Klein O. I., Pivchenko D. V. et al. Oil degradation by basidiomycetes in soil and peat at low temperatures. Appl. Biochem. Microbiol. 2016. V. 52 (6). P. 629–637. https://doi.org/10.1134/S0003683816060119
  21. Kumar R., Singh S., Singh O. V. Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives. J. Industrial Microbiol. Biotechnol. 2008. V. 35 (5). P. 377–391.
  22. Kumla J., Suwannarach N., Sujarit K. et al. Cultivation of mushrooms and their lignocellulolytic enzyme production through the utilization of agro-industrial waste. Molecules. 2020. V. 25 (12). https://doi.org/10.3390/molecules25122811
  23. Li I. C., Lee L. Y., Tzeng T. T. et al. Neurohealth properties of Hericium erinaceus mycelia enriched with erinacines. Behavioural Neurology. 2018. V. 2018. Art. 5802634. https://doi.org/10.1155/2018/5802634
  24. Lodge D. J., Ammirati J. F., O’Dell T.E. et al. Terrestrial and lignicolous macrofungi. In: G. M. Mueller, G. F. Bills, M. S. Foster (eds). Biodiversity of Fungi: inventory and monitoring methods. 1st edn. Elsevier Academic Press, Amsterdam, 2004, pp. 127–172.
  25. Mattila P., Könkö K., Eurola M. et al. Contents of vitamins, mineral elements, and some phenolic compounds in cultivated mushrooms. J. Agric. Food Chemistry. 2001. V. 49 (5). P. 2343–2348. https://doi.org/10.1021/jf001525d
  26. Ministry of Natural Resources and Environment of the Russian Federation [Electronic resource]. https://www.mnr.gov.ru. Accessed 01.12.2024.
  27. Mueller G. M., Schmit J. P., Leacock P. R. et al. Global diversity and distribution of macrofungi. Biodiversity and Conservation. 2007. V. 16. P. 37–48. https://doi.org/10.1007/s10531-006-9108-8
  28. Newman D. The distribution of range in samples from a normal population, expressed in terms of an independent estimate of standard deviation. Biometrika. 1939. V. 31 (1/2). P. 20–30. https://doi.org/10.2307/2334973
  29. Onions A. H.S. Preservation of Fungi. In: C. Booth (ed.). Methods in microbiology. V. 4. Academic Press, L., 1971, pp. 113–151.
  30. Ozerskaya S. M., Kochkina G. A., Ivanushkina N. E., Zaprometova K. M., Eremina S. S., Knyazeva E. V. State of microorganism collections in Russia. Bulletin of Biotechnology and Physical-Chemical Biology named after Yu. A. Ovchinnikov. 2006. V. 2 (3). P. 51–61. (In Russ.).
  31. Patel Y., Naraian R., Singh V. K. Medicinal properties of Pleurotus species (Oyster Mushroom): A review. World J. Fungal Plant Biol. 2012. V. 3 (1). P. 1–12.
  32. Psurtseva N. V., Kiyashko A. A. Scientific and practical potential of the fungal gene pool in the collection of basidiomycete cultures of the Botanical Institute of the Russian Academy of Sciences // Collections as a basis for studying the genetic resources of plants and fungi: Abstracts of reports of the All-Russian conference. St. Petersburg, June 22–23, 2022. Komarov Botanical Institute of the Russian Academy of Sciences, St. Petersburg, 2022, p. 42. (In Russ.).
  33. Red data book of the Russian Federation. Plants and Fungi (ed. D. V. Geltman). VNII “Ekologiya”, Moscow, 2024. (In Russ.).
  34. Rodríguez-Rodríguez C.E., Castro-Gutiérrez V., Chin- Pampillo J.S. et al. On-farm biopurification systems: role of white rot fungi in depuration of pesticide-containing wastewaters. FEMS Microbiol. Letters. 2013. V. 345 (1). https://doi.org/10.1111/1574-6968.12161
  35. Rosales E., Pazos M., Ángeles Sanromán M. Feasibility of solid-state fermentation using spent fungi-substrate in the biodegradation of PAHs. CLEAN – Soil, Air, Water. 2013. V. 41 (6). P. 610–615. https://doi.org/10.1002/clen.201100305
  36. Sinitsyn A. P., Gusakov A. V., Chernoglazov V. M. Bioconversion of lignocelluloses materials: Textbook. Moscow State University Publishing House, Moscow, 1995. (In Russ.).
  37. Stamets P. Mycelium running: How mushrooms can help save the world. Ten speed press, Berkeley, 2005.
  38. Tan M., Mei J., Xie J. The formation and control of ice crystal and its impact on the quality of frozen aquatic products: A review. Crystals. 2021. V. 11 (1). https://doi.org/10.3390/cryst11010068
  39. Teeri T. T. Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Trends Biotechnol. 1997. V. 15 (5). P. 160–167.
  40. Tukey J. W. Comparing individual means in the analysis of variance. Biometrics. 1949. V. 5 (2). P. 99–114.
  41. Vetter J. Biological values of cultivated mushrooms – a review. Acta Alimentaria. 2019. V. 48 (2). P. 229–240. https://doi.org/10.1556/066.2019.48.2.11
  42. Xia J., Dai L., Wang L. et al. Ganoderic acid DM induces autophagic apoptosis in non-small cell lung cancer cells by inhibiting the PI3K/Akt/mTOR activity. ChemicoBiological Interactions. 2020. V. 316. https://doi.org/10.1016/j.cbi.2019.108932
  43. Антропова А. Б., Белозерская Т. А., Белозерский М. А. и др. (Antropova et al.) Практикум по физиологии и биохимии грибов. Уч. пособие (ред. А. В. Кураков). М.: Изд. Биол. ф-т МГУ, 2017. 215 с.
  44. Бухало А. С. (Bukhalo) Высшие съедобные базидиомицеты в чистой культуре. Киев: Наук. думка, 1988. 143 с.
  45. Коваленко А. Е. (Kovalenko) Экологический обзор грибов из порядков Polyporales s.str., Boletales, Agaricales s. str., Russulales в горных лесах центральной части Северо-Западного Кавказа // Микология и фитопатология. 1980. Т. 14. № 4. С. 300–314.
  46. Коваленко С. А. (Kovalenko) Коллекционный фонд штаммов базидиомицетов Института леса НАН Беларуси // Проблемы лесной фитопатологии и микологии: материалы XI международной конференции, Петрозаводск, 10–14 октября 2022 года. Петрозаводск: КарНЦ РАН, 2022. С. 26–28.
  47. Комиссаров Н. С., Дьяков М. Ю., Гарибова Л. В. (Komissarov et al.) Методы длительного хранения чистых культур макромицетов // Микология и фитопатология. 2023. Т. 57. № 3. С. 155–171.
  48. Красная книга Российской Федерации. Растения и грибы (Red data book) Москва: ВНИИ “Экология”, 2024. 944 с.
  49. Министерство природных ресурсов и экологии Российской Федерации [Электронный ресурс] (Ministry). https://www.mnr.gov.ru.
  50. Озерская С. М., Кочкина Г. А., Иванущкина Н. Е. и др. (Ozerskaya et al.) Состояние коллекций микроорганизмов в России // Вестник биотехнологии и физико-химической биологии имени Ю. А. Овчинникова. 2006. Т. 2. № . 3. С. 51–61.
  51. Псурцева Н. В., Кияшко А. А. (Psurtseva, Kiyashko) Научно-практический потенциал генофонда грибов в коллекции культур базидиомицетов БИН РАН // Коллекции как основа изучения генетических ресурсов растений и грибов: Тезисы докладов Всероссийской конференции. Санкт-Петербург, 22–23 июня 2022 г. СПб.: Ботанический институт им. В. Л. Комарова РАН, 2022. C. 42.
  52. Синицын А. П., Гусаков А. В., Черноглазов В. М. (Sinitsyn et al.) Биоконверсия лигноцеллюлозных материалов: Уч. пособие. М.: Изд-во МГУ, 1995. 224 с.

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