Novacutan preparations as potential dermatogeroprotectors: molecular-microscopic study


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Abstract

Introduction. The work is devoted to study of new medication Novacutan SBio and Novacutan YBio as potential geroprotectors for biorevitalization of the skin. Klotho was used as a marker - to assess the overall aging of cells; MTH1 - the antioxidant status of cells; AR-1 - U.V. resistance of cells; synthetic activity of cells: elastin and collagen. The aim of the study was to compare the geroprotective properties of Novacutan YBio and Novacutan SBio. Methods. To study the aging of skin cells in culture, we used the method of combined cultivation of fibroblasts and keratinocytes of human skin, and to verify the expression of signaling molecules - aging markers - methods of immunocytochemistry and confocal microscopy. An in vitro experiment was performed to study of biopsies of the skin of patients after applying various therapies using Novacutan preparations. Results. We studied the expression of signaling molecules/markers AP-1, Klotho, Elastin, MTH-1 in samples of “young” and «old» cell cultures of human fibroblasts and keratinocytes when exposed to them with Novacutan YBio and Novacutan SBio. The authors showed a stimulating effect of Novacutan on the expression of markers Klotho, Elastin, MTH-1, and inhibitory effect on AP-1 expression during cell aging in cultures. Under in vitro conditions. Novacutan Y and S preparations were found to activate synthetic processes in the skin. In this case, the combination therapy of laser skin resurfacing with Novacutan biorevitalization courses is the most favorable. Conclusion. These studies allow considering the preparation Novacutan as a drug with pronounced general regulatory and dermatogeroprotective properties.

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About the authors

Zarema Ibragimovna Gazitaeva

Institute of Beauty Fijie; St. Petersburg Institute of Bioregulation and Gerontology Russian Federation

Email: gaz.itaevadoctor@gmail.com
Head of the Department of Medical Support

Anna Olegovna Drobintseva

St. Petersburg State Pediatric Medical University

Email: anna.drobintseva@gmail.com
assistant professor. Department of medical biology.

Victoria Olegovna Polyakova

St. Petersburg State Pediatric Medical University

Email: vopol@yandex.ru
head of research center

Igor Moiseevich Kvetnoy

Saint-Petersburg Federal Research Institute of Phthisiology and Pulmonology, Healthcare Ministry of Russia

Email: igor.kvetnoy@yandex.ru
head of molecular biomedicine center

Aleksei Yurevich Prokopov

ROS-Chimia Ltd

Email: a.prokopov@ros-chimia.ru
director

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