DETERMINATION OF FORMALDEHYDE, GLYOXAL, GLUTARALDEHYDE, AND o-PHTHALALDEHYDE IN THE PRESENCE OF EACH OTHER IN DISINFECTANTS USING 2,4-DINTROPHENYLHYDRAZINE

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Abstract

Dialdehydes are predominantly used as disinfectants because these compounds exhibit a broad spectrum of antimicrobial activity. Among monoaldehydes, formaldehyde is used to a limited extent due to its higher toxicity. This work describes conditions for the simultaneous derivatization of formaldehyde, glyoxal, glutaraldehyde, and o-phthalaldehyde with 2,4-dinitrophenylhydrazine. The reaction is carried out in an acetonitrile–methanol mixture at 50°C in an ultrasonic bath using trifluoroacetic acid as a catalyst. The best separation of mixture components was achieved on a C18 column in gradient elution mode with acetonitrile and acetate buffer solution (pH 5.4) at a variable flow rate. The linearity range for formaldehyde was 2.51–20.0 mg/L, for glutaraldehyde 4.92–21.9 mg/L, for o-phthalaldehyde 1.98–6.94 mg/L, and for glyoxal 2.00–10.0 mg/L. The limits of detection for formaldehyde, glyoxal, glutaraldehyde, and o-phthalaldehyde were 0.453, 0.177, 0.967, and 0.760 mg/L, respectively. The developed method was successfully applied for the simultaneous determination of aldehydes in disinfectants.

About the authors

A. D Salimova

Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing

Moscow, Russia

S. V Andreev

Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing; Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University

Email: svandreev.niid@gmail.com
Moscow, Russia; Moscow, Russia

L. Yu Martynov

Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University

Moscow, Russia

A. A Ischenco

Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University

Moscow, Russia

R. D Solovov

Institute of Disinfectology of the Federal Scientific Center of Hygiene named after F. F. Erisman of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing; Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University

Moscow, Russia; Moscow, Russia

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