Influence of separate and combined action of γ-radiation and lead nitrate on germination, antioxidant status and cytogenetic indicators of spring barley seedlings

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Exposure of seeds to a dose of γ-radiation of 20 Gy and a concentration of 2 mg/ml lead significantly modifies the oxidative status of barley plants. Lead at the concentration studied is capable of suppressing the development of seedlings, but is inferior to γ-radiation in its ability to induce cytogenetic abnormalities in the root meristem. Lead toxicity affects roots much more than shoots because roots interact directly with the lead solution. Preliminary seeds γ-irradiation modifies the oxidative status of plants, which reduces the negative effect of lead on seed germination and mitotic activity of cells. The combined action of lead and ionizing radiation does not lead to increased oxidative stress and an increase in the frequency of cytogenetic abnormalities compared to their separate action.

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作者简介

Stanislav Geras’kin

National Research Center “Kurchatov Institute” – RIRAE

编辑信件的主要联系方式.
Email: stgeraskin@gmail.com
ORCID iD: 0000-0001-9978-3049
俄罗斯联邦, Obninsk

Alexander Prazyan

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
俄罗斯联邦, Obninsk

Denis Vasiliev

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0003-0463-0029
俄罗斯联邦, Obninsk

Sofia Bitarishvili

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0002-3623-7128
俄罗斯联邦, Obninsk

Alena Smirnova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0009-0006-1263-9188
俄罗斯联邦, Obninsk

Ekaterina Shesterikova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0001-6737-5753
俄罗斯联邦, Obninsk

Anastasiya Khanova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0003-1171-0844
俄罗斯联邦, Obninsk

Ivan Pishenin

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0002-2633-9251
俄罗斯联邦, Obninsk

Elizaveta Kazakova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0002-2975-5891
俄罗斯联邦, Obninsk

Ekaterina Kvichanskaia

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0001-7519-9550
俄罗斯联邦, Obninsk

Mariya Lychenkova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0001-6148-2021
俄罗斯联邦, Obninsk

Darya Babina

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0003-2544-9667
俄罗斯联邦, Obninsk

Marina Korol

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0003-0911-2650
俄罗斯联邦, Obninsk

Yana Blinova

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
俄罗斯联邦, Obninsk

Mikhail Podlutskii

National Research Center “Kurchatov Institute” – RIRAE

Email: stgeraskin@gmail.com
ORCID iD: 0000-0002-7890-0469
俄罗斯联邦, Obninsk

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2. Fig. 1. Conditions for barley germination using the roll method for biochemical studies (A) and in Petri dishes for cytogenetic analysis (B).

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3. Fig. 2. Catalase activity in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test.

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4. Fig. 3. Activity of ascorbate peroxidase in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test. + Significant relative to the combined effect of factors, p < 0.05, Mann–Whitney U-test.

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5. Fig. 4. Activity of guaiacol peroxidase in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead.

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6. Fig. 5. Concentrations of ascorbic acid in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test. + Significant relative to irradiated plants, p < 0.05, Mann–Whitney U-test.

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7. Fig. 6. Concentrations of oxidized glutathione in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. ▼ Significant relative to irradiated plants, p < 0.05, Mann–Whitney U-test.

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8. Fig. 7. Concentrations of reduced glutathione in sprouts (A) and roots (B) of 7-day-old barley seedlings after separate and combined exposure to γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test. ▼ Significant relative to irradiated plants, p < 0.05, Mann–Whitney U-test.

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9. Fig. 8. Concentrations of MDA in shoots (A) and roots (B) of 7-day-old barley seedlings after separate and combined effects of γ-radiation and lead. * Significant relative to control, p < 0.05, Mann–Whitney U-test. ▼ Significant relative to irradiated plants, p < 0.05, Mann–Whitney U-test.

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10. Fig. 9. Seed germination (A) and mitotic index in cells of the root meristem of barley seedlings (B) on the 7th day of germination after separate and combined action of γ-radiation and lead salt. * Difference from control is statistically significant, p < 0.05.

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