Clinical studies of the human microbiome. Strategies for applying methods and translating results into clinical practice: A review
- 作者: Koshechkin S.I.1, Odintsova V.E.1, Karasev A.V.2, Zakharova I.N.3, Berezhnaya I.V.3, Pervishko O.V.4, Kuchina A.E.3, Yudina A.E.5, Kuznetsova I.S.3, Dmitrieva D.K.3, Orobinskaya Y.V.3, Serikova L.S.6, Makhaeva A.V.3,7, Romanov V.A.1
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隶属关系:
- Nobias Technologies LLC
- Laboratory "ABT"
- Russian Medical Academy of Continuous Professional Education
- Kuban State Medical University
- Bauman City Clinical Hospital №29
- National Medical Research Center for High Medical Technologies – Vishnevsky Central Military Clinical Hospital
- Children's City Polyclinic №140
- 期: 编号 1 (2024)
- 页面: 15-24
- 栏目: Articles
- URL: https://pediatria.orscience.ru/2658-6630/article/view/633321
- DOI: https://doi.org/10.26442/26586630.2024.1.202774
- ID: 633321
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Current omics methods, which are essential for scientific research, are overviewed. These methods enable studies of the mechanisms of clinical manifestations by analyzing the relationship between the microbiota characteristics and clinical parameters. To represent the complex interactions between the microbiome and host metabolism, metagenomics and metabolomics techniques that contribute to the search for new therapeutic approaches are most relevant. Based on the metagenomic data, the associated taxa are searched, and the metabolic profile indicates the result of the activity of the microbial community. The characteristics of technologies for studying metagenomes using the amplicon sequencing method are considered, and the depth of identification of microorganisms, the level of sequencing errors and the preference in terms of cost are evaluated. Studying the evolution of pathogens and metabolic processes, expressed genes, and determinants of antibiotic resistance contributes to the development of rational strategies for disease therapy and control of the spread of infectious diseases. In recent years, the number of scientific projects in the field of microbiota research has been steadily increasing, which necessitates the need to raise physicians' awareness of modern methods and research approaches in order to apply relevant data in practical work.
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作者简介
Stanislav Koshechkin
Nobias Technologies LLC
编辑信件的主要联系方式.
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-7389-0476
Cand. Sci. (Med.)
俄罗斯联邦, Moscow
Vera Odintsova
Nobias Technologies LLC
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0003-1897-4033
Сhief bioinformatician
俄罗斯联邦, MoscowAlexander Karasev
Laboratory "ABT"
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0001-7484-4992
Chief Executive Officer
俄罗斯联邦, MoscowIrina Zakharova
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0003-4200-4598
Sci. (Med.), Prof.
俄罗斯联邦, Moscow
Irina Berezhnaya
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-2847-6268
Cand. Sci. (Med.)
俄罗斯联邦, MoscowOlesya Pervishko
Kuban State Medical University
Email: ole-pervishko@yandex.ru
ORCID iD: 0000-0003-1083-2807
Cand. Sci. (Med.), Assoc. Prof.
俄罗斯联邦, KrasnodarAnastasiya Kuchina
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-8998-264X
pediatrician
俄罗斯联邦, Moscow
Anastasiya Yudina
Bauman City Clinical Hospital №29
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-6920-8024
Department Head
俄罗斯联邦, MoscowIrina Kuznetsova
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0001-5164-682X
Assistant
俄罗斯联邦, MoscowDiana Dmitrieva
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-1593-0732
Graduate Student
俄罗斯联邦, MoscowYana Orobinskaya
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0009-0005-2121-4010
Graduate Student
俄罗斯联邦, MoscowLiudmila Serikova
National Medical Research Center for High Medical Technologies – Vishnevsky Central Military Clinical Hospital
Email: St.Koshechkin@gmail.com
pediatrician
俄罗斯联邦, MoscowAnastasia Makhaeva
Russian Medical Academy of Continuous Professional Education; Children's City Polyclinic №140
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-0006-5889
Graduate Student, Russian Medical Academy of Continuous Professional Education, pediatrician, Department Head, Children's City Polyclinic №140
俄罗斯联邦, Moscow; MoscowVladimir Romanov
Nobias Technologies LLC
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-7540-5884
clinical research manager
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