Clinical studies of the human microbiome. Strategies for applying methods and translating results into clinical practice: A review
- Authors: Koshechkin S.I.1, Odintsova V.E.1, Karasev A.V.2, Zakharova I.N.3, Berezhnaya I.V.3, Pervishko O.V.4, Kuchina A.E.3, Yudina A.E.5, Kuznetsova I.S.3, Dmitrieva D.K.3, Orobinskaya Y.V.3, Serikova L.S.6, Makhaeva A.V.3,7, Romanov V.A.1
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Affiliations:
- Nobias Technologies LLC
- Laboratory "ABT"
- Russian Medical Academy of Continuous Professional Education
- Kuban State Medical University
- Bauman City Clinical Hospital №29
- National Medical Research Center for High Medical Technologies – Vishnevsky Central Military Clinical Hospital
- Children's City Polyclinic №140
- Issue: No 1 (2024)
- Pages: 15-24
- Section: Articles
- URL: https://pediatria.orscience.ru/2658-6630/article/view/633321
- DOI: https://doi.org/10.26442/26586630.2024.1.202774
- ID: 633321
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Abstract
Current omics methods, which are essential for scientific research, are overviewed. These methods enable studies of the mechanisms of clinical manifestations by analyzing the relationship between the microbiota characteristics and clinical parameters. To represent the complex interactions between the microbiome and host metabolism, metagenomics and metabolomics techniques that contribute to the search for new therapeutic approaches are most relevant. Based on the metagenomic data, the associated taxa are searched, and the metabolic profile indicates the result of the activity of the microbial community. The characteristics of technologies for studying metagenomes using the amplicon sequencing method are considered, and the depth of identification of microorganisms, the level of sequencing errors and the preference in terms of cost are evaluated. Studying the evolution of pathogens and metabolic processes, expressed genes, and determinants of antibiotic resistance contributes to the development of rational strategies for disease therapy and control of the spread of infectious diseases. In recent years, the number of scientific projects in the field of microbiota research has been steadily increasing, which necessitates the need to raise physicians' awareness of modern methods and research approaches in order to apply relevant data in practical work.
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About the authors
Stanislav I. Koshechkin
Nobias Technologies LLC
Author for correspondence.
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-7389-0476
Cand. Sci. (Med.)
Russian Federation, Moscow
Vera E. Odintsova
Nobias Technologies LLC
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0003-1897-4033
Сhief bioinformatician
Russian Federation, MoscowAlexander V. Karasev
Laboratory "ABT"
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0001-7484-4992
Chief Executive Officer
Russian Federation, MoscowIrina N. Zakharova
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0003-4200-4598
Sci. (Med.), Prof.
Russian Federation, Moscow
Irina V. Berezhnaya
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-2847-6268
Cand. Sci. (Med.)
Russian Federation, MoscowOlesya V. Pervishko
Kuban State Medical University
Email: ole-pervishko@yandex.ru
ORCID iD: 0000-0003-1083-2807
Cand. Sci. (Med.), Assoc. Prof.
Russian Federation, KrasnodarAnastasiya E. Kuchina
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-8998-264X
pediatrician
Russian Federation, Moscow
Anastasiya E. Yudina
Bauman City Clinical Hospital №29
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-6920-8024
Department Head
Russian Federation, MoscowIrina S. Kuznetsova
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0001-5164-682X
Assistant
Russian Federation, MoscowDiana K. Dmitrieva
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-1593-0732
Graduate Student
Russian Federation, MoscowYana V. Orobinskaya
Russian Medical Academy of Continuous Professional Education
Email: St.Koshechkin@gmail.com
ORCID iD: 0009-0005-2121-4010
Graduate Student
Russian Federation, MoscowLiudmila S. Serikova
National Medical Research Center for High Medical Technologies – Vishnevsky Central Military Clinical Hospital
Email: St.Koshechkin@gmail.com
pediatrician
Russian Federation, MoscowAnastasia V. Makhaeva
Russian Medical Academy of Continuous Professional Education; Children's City Polyclinic №140
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-0006-5889
Graduate Student, Russian Medical Academy of Continuous Professional Education, pediatrician, Department Head, Children's City Polyclinic №140
Russian Federation, Moscow; MoscowVladimir A. Romanov
Nobias Technologies LLC
Email: St.Koshechkin@gmail.com
ORCID iD: 0000-0002-7540-5884
clinical research manager
Russian Federation, MoscowReferences
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