Получение 6-азидо-2-хлорпурин-2′-дезоксирибозида с помощью реакции ферментативного трансгликозилирования, катализируемой нуклеозиддезоксирибозилтрансферазой второго типа Lactobacillus leichmannii

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Аннотация

6-azido-2-chloropurine-2′-deoxyriboside, a valuable precursor for the preparation of modified 2-chloropurine nucleosides substituted at the 6-position of the heterocyclic base, was obtained by enzymatic transglycosylation. 6-azido-2-chloropurine-2′-deoxyriboside can also be used as a photocross-linking agent to study the nucleic acids – proteins interactions. A type 2 nucleoside deoxyribosyltransferase from Lactobacillus leichmannii was used as a biocatalyst. The optimal conditions for the formation of 6-azido-2-chloropurine-2′-deoxyriboside using 7-methyl-2′-deoxyguanosine as a carbohydrate residue donor were determined.

Об авторах

К. С. Алексеев

Институт молекулярной биологии им. В. А. Энгельгардта Российской академии наук

Email: micelle@mail.ru
ул. Вавилова, 32, Москва, 119991 Россия

М. А. Конкина

Институт молекулярной биологии им. В. А. Энгельгардта Российской академии наук; Институт тонких химических технологий имени М. В. Ломоносова, “МИРЭА – Российский технологический университет”

Email: cyril.alex@eimb.ru
ул. Вавилова, 32, Москва, 119991 Россия; проспект Вернадского, д. 86, Москва, 119571 Россия

Н. Н. Курочкин

Институт молекулярной биологии им. В. А. Энгельгардта Российской академии наук

Email: micelle@mail.ru
ул. Вавилова, 32, Москва, 119991 Россия

М. С. Дреничев

Институт молекулярной биологии им. В. А. Энгельгардта Российской академии наук

Автор, ответственный за переписку.
Email: micelle@mail.ru
ул. Вавилова, 32, Москва, 119991 Россия

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