A SYNGENIC MOUSE MODEL OF BREAST CANCER EXPRESSING HUMAN ERBB2 AND NANOLUC LUCIFERASE GENES

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Abstract

Syngenetic models are widely used in experimental oncology both for modeling tumor diseases and for testing anticancer drugs. However, when testing targeted drugs aimed at human tumor-associated antigens, the presence of target antigens in the animal's body is important. In this work, a syngenetic cell line with stable expression of two genes — ERBB2 gene encoding human epidermal growth factor type 2 HER2, and NaoLuc luciferase gene — was created based on human murine mammary gland carcinoma. Optical bioimaging methods have proven that the created cell line is characterized by stable expression of ERBB2 and NanoLuc in vitro and in vivo, retains the aggressiveness growth of the original 4T1 cell line in animals, and forms spontaneous metastases that are detected in the animal's body by intravital biovisualization methods.

About the authors

E. I. Shramova

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry RAS; Moscow Institute of Engineering Physics, National Research Nuclear University "MEPhI"

Email: shramova.e.i@gmail.com
Moscow, Russian Federation; Moscow, Russian Federation

S. M. Deyev

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry RAS; Moscow Institute of Engineering Physics, National Research Nuclear University "MEPhI"; Ogarev National Research Mordovian State University

Academician of the RAS Moscow, Russian Federation; Moscow, Russian Federation; Saransk, Russian Federation

G. M. Proshkina

M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry RAS

Moscow, Russian Federation

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