STUDY OF CYTOTOXICITY OF FULLERENOL C60(OH)22-24 IN RELATION TO HUMAN PERIPHERAL BLOOD MONOCYTES

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详细

We studied the effect of fullerenol C60(OH)22–24 on the viability of human peripheral blood monocytes and its adhesion/internalization by these cells. Nanoparticles at concentrations of 0.25, 0.5, 2.5, 5, 12.5, 25, 50, 100, and 200 μg/mL were incubated with human blood mononuclear cells for 24, 48, and 72 hours (5% CO2 at 37°C). We subsequently determined monocyte viability and fullerenol fluorescence intensity in the monocyte gate using flow cytometry. Nanoparticles were adsorbed/internalized by cells proportionally to their concentration and incubation time. Fullerenol С60(ОН)22–24 at 200 μg/mL reduced monocyte viability by an average of 17%, 30%, and 28% after 24, 48, and 72 hours, respectively. Cell survival inversely correlated with nanoparticle uptake intensity. Concentrations ≤100 μg/mL did not exhibit cytotoxicity even after prolonged exposure, supporting its biomedical applicability.

作者简介

V. Timganova

Institute of Ecology and Genetics of Microorganisms

Email: timganovavp@gmail.com
branch of the Perm Federal Research Center Ural Branch Russian Academy of Sciences Perm, Russian Federation

M. Bochkova

Institute of Ecology and Genetics of Microorganisms; Perm State National Research University

branch of the Perm Federal Research Center Ural Branch Russian Academy of Sciences Perm, Russian Federation; Perm, Russian Federation

S. Lazarev

Institute of Ecology and Genetics of Microorganisms

branch of the Perm Federal Research Center Ural Branch Russian Academy of Sciences Perm, Russian Federation

M. Dolgikh

Institute of Ecology and Genetics of Microorganisms; Perm State National Research University

branch of the Perm Federal Research Center Ural Branch Russian Academy of Sciences Perm, Russian Federation; Perm, Russian Federation

D. Usanina

Institute of Ecology and Genetics of Microorganisms; Perm State National Research University

branch of the Perm Federal Research Center Ural Branch Russian Academy of Sciences Perm, Russian Federation; Perm, Russian Federation

S. Zamorina

Institute of Ecology and Genetics of Microorganisms; Perm State National Research University

branch of the Perm Federal Research Center Ural Branch Russian Academy of Sciences Perm, Russian Federation; Perm, Russian Federation

M. Rayev

Institute of Ecology and Genetics of Microorganisms; Perm State National Research University

branch of the Perm Federal Research Center Ural Branch Russian Academy of Sciences Perm, Russian Federation; Perm, Russian Federation

参考

  1. Пирунгин С.К., Туровецкий В.Б., Кедров А.В., и др. Влияние гидроксилированного фуллерена C60(OH)25 на целостность плазматических мембран макрофагов // Радиационная биология. Радиоэкология. 2012. T. 52. №3. C. 252–256.

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